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QIAGEN51185;51185;QIAamp DNA Blood Midi Kit (100);血液DNA Midi试剂盒(100T);For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
编号:51185
品牌:Qiagen   [查询该品牌全部商品]
单位:盒
★如需其它(规格/包装)请电询我司!
★QIAGEN51185
★血液DNA Midi试剂盒
★高品质的快速纯化,准备使用的DNA
无有机萃取或乙醇沉淀
一致的是,高收益
完全去除污染物和抑制剂的
的QIAamp DNA血南部套件针对处理全血,血浆,血清和其他体液的实验室提供的二氧化硅 - 膜 - 基于DNA纯化。该DNA的QIAamp血液Midi试剂盒是专为处理0.3-2毫升新鲜或冷冻人全血。所述的QIAamp南部旋转柱可以在离心机中或真空歧管易于加工(附加缓冲液AW1[目录号19081]和缓冲AW2[目录号19072]所需的真空歧管的使用)。
商品售价:¥7,895.00
订购数量:
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商品介绍
详细参数
Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").

The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery DNA, depending on the starting cell densities (see table).

DNA yields from human whole blood with different cell densities.
Leukocytes per ml DNA yield (µg) DNA recovery (%)
2.5 x 105 3.0 92.3
1.0 x 106 11.0 91.7
5.0 x 106 62.4 94.5
1.0 x 107 116.3 88.1
Features
Specifications
Applications PCR, southern blotting
Elution volume 100–400 µl
Format Spin column
Main sample type Whole blood, body fluids
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA, viral DNA
Sample amount 0.3–2 ml
Technology Silica technology
Time per run or per prep 55 minutes
Yield 20–60 µg
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